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LifeSensors - Expression Systems

EXPRESSION SYSTEMS - Yeast
Expression Kits

Yeast is a cost-effective alternative for the expression of proteins that require a eukaryotic system to facilitate folding, yet it does not necessarily require advanced post-translational modification(s) associated with mammalian systems. Intracellular expression of SUMOstar fusion proteins in Saccharomyces can be achieved with pY-SUMOstar. This vector allows for yeast strain selection with the Trp1 auxotrophic marker gene; it contains a high copy (2-micron) origin, and confers ampicillin resistance for selection in E. coli. Recombinant protein expression is driven by the tightly regulated easily induced yeast metallothionein CUP1 promoter, which is easily regulated by copper. The use of this vector requires Saccharomyces strains that are trp1 and not copper sensitive.

The methylotrophic yeast Pichia pastoris has gained popularity as an expression host for its ability to produce, in many cases, very large quantities of recombinant protein. The use of P. pastoris additionally benefits from its propensity for high density growth in minimal media, post-translational modifications (e.g. glycosylation), and efficient protein secretion. However, production of recombinant proteins in P. pastoris is highly variable and greatly influenced by the inherent properties of the protein of interest. Fusion to SUMO3 helps to eliminate this variability, and may greatly enhance protein production and purification of native protein. Expression and secretion of SUMO3 fusion proteins in P. pastoris is achieved with pP-SUMOpro3. Protein production is placed under the control of the strong, tightly regulated and methanol inducible alcohol oxidase promoter (AOX1).

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Expression Systems
Expression Systems
PRODUCT NAME CATALOG#        PRICE (USD) QTY
Expression Systems
Yeast Intracellular Expression (Saccharomyces)     More Information »
pY-Saccharomyces SUMOstar Intracellular Kit 2100
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Yeast Secretory Expression (Pichia)     More Information »
pP-Pichia Secretory SUMOpro3 Expression (LIC) Kit LIC2150 Please Inquire  
pP-Pichia Secretory SUMOpro3 Expression Kit 2150
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LifeSensors Launches DiUbiquitin Substrate
A Novel Fluorescent Assay for Ubiquitin Isopeptide Bond Cleavage
MALVERN, PA -- June 29, 2010 --LifeSensors, Inc., a biotechnology company, announces the launch of its novel physiologically relevant diubiquitin substrates for measuring isopeptide bond cleavage (patent applied for). This breakthrough technology, for both basic research and drug discovery targeting the ubiquitin proteasome pathway, offers sensitive, rapid, and robust fluorescent readouts of isopeptidase or de-ubquitylase (DUB) activity.
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LifeSensors

LifeSensors' Gender Sorting Technology Discussed
Feb 9th 2010 | From The Economist online
Dr Butt’s new device is an oestrogen sniffer. It relies on the fact that female embryos produce this hormone in quantity and male ones do not. The sensor uses a fine needle to penetrate both the shell and the allantoic sac of an egg. This sac is a fluid-filled membrane that cushions the embryo and helps it trade carbon dioxide for oxygen from the air. (It is also the membrane that can make peeling a hard-boiled egg such a frustrating affair.)
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LifeSensors

LifeSensors Publishes in Journal of Animal Science
2010 Jan 15.
An estrogen sensor for poultry gender sorting.

Tran HT, Ferrell W, Butt TR.

The need for segregation of poultry based on sex is driven by gender-related differences in growth rate, market age, management practices, and nutritional requirements. Each day, global poultry industry staff would ideally like to determine the gender of >150 million newly hatched birds. Currently, this can be done only manually at the hatchery, which is a virtually impossible undertaking. LifeSensors has developed a facile, rapid, and low cost yeast-based assay that distinguishes male from female embryonated eggs before hatching based on the estrogen level of their allantoic fluid.
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