LifeSensors has developed TUBE (Tandem Ubiquitin Binding Entity) technology for the characterization and isolation of polyubiquitylated proteins from cells and tissue extracts.  TUBEs have 100 to 1000-fold higher affinity for polyUb chains compared to monomer UBDs. TUBEs both stabilize and bind to ubiquitylated proteins, serving as an indispensable tool for ubiquitologists.

The conjugation of ubiquitin to substrates is a multi-step, multi-enzyme process. LifeSensors is committed to supplying high throughput, facile, and robust assays for measuring the activity of Ub conjugating enzymes. These assays work with a wide range of E3 ligases including members of the three major classes of E3 ligases (simple RING class, SCF RING class, and HECT class).  Our E3LITE Customizable Ubiquitin Ligase Kit, as well as the E2 profiling kit based on it, was developed in collaboration with and licensed from Progenra, Inc. (www.progenra.com).

Ubiquitin/Ubl conjugating enzymes are responsible for the selective modification of substrates. LifeSensors supplies a wide assortment of these enzymes to aid researchers in their efforts to understand the specificity and selectivity of this complex enzyme pathway.

Ub/Ubl isopeptidases play key regulatory roles in a variety of disease states including cancer, diabetes, and infectious disease. As such, they represent an attractive class of novel targets for therapeutic development. Approximately 100 Ub/Ubl isopeptidases have been identified in the human genome, however relatively few have been fully characterized. LifeSensors' offers a wide range of HTS amenable assays substrates for characterizing these proteases and discovering modulators of their activity.

LifeSensors has the largest collection of Ub/Ubl isopeptidases commercially available. These enzymes can be utilized for HTS, determining Ub/Ubl specificity or Ub chain linkage type, as well as target substrate specificity.

There is a known causative link between the ubiquitin system and many human diseases including cancer, neurodegeneration, metabolic disorders and viral infections. Proteolysis mediated by the ubiquitin-proteasome system is regulated by different enzymes, which recently became an attractive target for drug discoveries. LifeSensors offers a number of inhibitors enabling researchers to decipher the function of Ub/Ubl isopeptidases and conjugating enzymes. Along with classical inhibitors of Ub/Ubl isopeptidases such as Ub-vinyl methyl ester and –vinyl sulfone, LifeSensors has a number of small molecule inhibitors. Our new addition includes inhibitors of Ub/Ubl conjugating enzymes. With an evergrowing interest in proteasomal activity that has been linked to cancer, inflammatory and autoimmune diseases, we now offer a collection of proteasome inhibitors. Several inhibitors including bortezomib are already used in the treatment of multiple myeloma. The most commonly used laboratory inhibitors, lactacystin and MG132 are now available in different quantities.

Antibodies can be used in a wide range of applications including Western blotting, ELISA assays, immunofluorescence and immunoprecipitation. LifeSensors offers quality antibodies recognizing many of the enzymes involved in protein ubiquitylation to aid researchers in their quest to uncover novel protein functions.

The world's most versatile and informative microarray, dedicated to the deconjugation side of the ubiquitin (and Ubl) pathways(s). Select one of three application protocols for use with either DUB proteins, DUB antibodies, or catalytically active DUB enzymes. Identify DUBs for proteins of interest, discover biomarkers, or screen DUB inhibitors in a multiplexed fashion.